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goat anti rabbit biotinylated secondary antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno goat anti rabbit biotinylated secondary antibody
    Goat Anti Rabbit Biotinylated Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+rabbit+secondary+antibody/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Rabbit+IgG%2C+Fc+fragment+specific/pmc12996694-111-14-22
    Average 93 stars, based on 34 article reviews
    goat anti rabbit biotinylated secondary antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Astrocyte activation in the cat dLGN following monocular retinal inactivation.
    Article Snippet: .. A rabbit recombinant primary antibody was used to target NeuN (1:1000)(ab177487; Abcam, USA; RRID:AB_2532109), and the secondary antibody used for NeuN labeling was a biotinylated goat anti-rabbit secondary antibody (1:500) (111–065-003; Jackson ImmunoResearch, USA). .. The cross-sectional profiles of neuron somata were revealed in separate sections of dLGN that were mounted onto glass slides and stained for Nissl substance with a 1 % Cresyl Violet solution (ab246817; Abcam, USA).

    Labeling:

    Article Title: Astrocyte activation in the cat dLGN following monocular retinal inactivation.
    Article Snippet: .. A rabbit recombinant primary antibody was used to target NeuN (1:1000)(ab177487; Abcam, USA; RRID:AB_2532109), and the secondary antibody used for NeuN labeling was a biotinylated goat anti-rabbit secondary antibody (1:500) (111–065-003; Jackson ImmunoResearch, USA). .. The cross-sectional profiles of neuron somata were revealed in separate sections of dLGN that were mounted onto glass slides and stained for Nissl substance with a 1 % Cresyl Violet solution (ab246817; Abcam, USA).

    Membrane:

    Article Title: Porphyromonas gingivalis exacerbates experimental autoimmune encephalomyelitis by driving Th1 differentiation via ZAP70/NF-κB signaling
    Article Snippet: The membrane was blocked with 5% BSA in TBST for 1.5 h at 37°C, then incubated for 2 h at 37°C with primary antibodies diluted in TBST with 5% BSA, including: NF-κB p65 (Rabbit anti-NF-κB p65, 32536,Abcam; 1:1000), phospho-NF-κB p65 (Ser276/Ser536,76302, Abcam; 1:1000), phospho-IkBα (Ser176/180; AP0707,ABclonal; 1:1000), IkBα (Rabbit anti-IkBα, 4814S,Cell Signaling Technology; 1:1000), and housekeeping proteins β-actin (total protein control, Rabbit anti-β-actin, 700068-8F10,Zheng Neng; 1:5000) and Histone H3 (nuclear control, Mouse anti-H3, 250182, Zheng Neng; 1:1000). .. After washing, the membrane was incubated with a biotinylated goat anti-rabbit secondary antibody [Biotin-SP AffiniPure Goat Anti-Rabbit IgG (H + L), Jackson ImmunoResearch, USA; 1:200,000] for 1 h at 37°C, followed by 0.1 μg/mL peroxidase-conjugated streptavidin (Jackson ImmunoResearch, USA). .. Detection was performed using ECL chemiluminescence reagents (Amersham/GE Healthcare), and images were captured with a Vilber Lourmat TFX-35 WL transilluminator (France).

    Article Title: Porphyromonas gingivalis exacerbates experimental autoimmune encephalomyelitis by driving Th1 differentiation via ZAP70/NF-κB signaling.
    Article Snippet: The membrane was blocked with 5% BSA in TBST for 1.5 h at 37°C, then incubated for 2 h at 37°C with primary antibodies diluted in TBST with 5% BSA, including: NF-kB p65 (Rabbit anti-NF-kB p65, 32536,Abcam; 1:1000), phospho-NF-kB p65 (Ser276/Ser536,76302, Abcam; 1:1000), phospho-IkBa (Ser176/180; AP0707,ABclonal; 1:1000), IkBa (Rabbit anti-IkBa, 4814S,Cell Signaling Technology; 1:1000), and housekeeping proteins b-actin (total protein control, Rabbit anti-b-actin, 700068-8F10,Zheng Neng; 1:5000) and Histone H3 (nuclear control, Mouse anti-H3, 250182, Zheng Neng; 1:1000). .. After washing, the membrane was incubated with a biotinylated goat anti-rabbit secondary antibody [Biotin-SP AffiniPure Goat AntiRabbit IgG (H + L), Jackson ImmunoResearch, USA; 1:200,000] for Frontiers in Immunology 05 1 h at 37°C, followed by 0.1 μg/mL peroxidase-conjugated streptavidin (Jackson ImmunoResearch, USA). .. Detection was performed using ECL chemiluminescence reagents (Amersham/GE Healthcare), and images were captured with a Vilber Lourmat TFX-35 WL transilluminator (France).

    Incubation:

    Article Title: Porphyromonas gingivalis exacerbates experimental autoimmune encephalomyelitis by driving Th1 differentiation via ZAP70/NF-κB signaling
    Article Snippet: The membrane was blocked with 5% BSA in TBST for 1.5 h at 37°C, then incubated for 2 h at 37°C with primary antibodies diluted in TBST with 5% BSA, including: NF-κB p65 (Rabbit anti-NF-κB p65, 32536,Abcam; 1:1000), phospho-NF-κB p65 (Ser276/Ser536,76302, Abcam; 1:1000), phospho-IkBα (Ser176/180; AP0707,ABclonal; 1:1000), IkBα (Rabbit anti-IkBα, 4814S,Cell Signaling Technology; 1:1000), and housekeeping proteins β-actin (total protein control, Rabbit anti-β-actin, 700068-8F10,Zheng Neng; 1:5000) and Histone H3 (nuclear control, Mouse anti-H3, 250182, Zheng Neng; 1:1000). .. After washing, the membrane was incubated with a biotinylated goat anti-rabbit secondary antibody [Biotin-SP AffiniPure Goat Anti-Rabbit IgG (H + L), Jackson ImmunoResearch, USA; 1:200,000] for 1 h at 37°C, followed by 0.1 μg/mL peroxidase-conjugated streptavidin (Jackson ImmunoResearch, USA). .. Detection was performed using ECL chemiluminescence reagents (Amersham/GE Healthcare), and images were captured with a Vilber Lourmat TFX-35 WL transilluminator (France).

    Article Title: Chronic electroconvulsive shock disrupts hippocampal physiology and impairs associative memory in mice
    Article Snippet: .. For free floating fluorescent immunostaining of c-fos, sections were incubated with 1% H 2 O 2 for 30min, rabbit anti-Fos antibody (1:1000; Synaptic Systems) for 3 days at 4°C, biotinylated goat anti-rabbit secondary antibody (Jackson Immunoresearch; 1:250) for 1 hour at room temperature, streptavidin-HRP for 1 hour and finally streptavidin-conjugated Alexa 647 (Thermofisher). ..

    Article Title: Active osseointegration in an ex vivo porcine bone model
    Article Snippet: All sections were rinsed the following day in wash buffer solution (pH 7.4; 3 × 5 min), which was composed of 1% Triton X-100 (Millipore 648643) in PBS. .. Sections were then incubated in biotinylated goat anti-rabbit secondary antibody (Jackson ImmunoResearch 111–066-046) at 1:250 PBS dilution for 1 h at room temperature, followed by rinses in the wash buffer (3 × 5 min). ..

    Article Title: Porphyromonas gingivalis exacerbates experimental autoimmune encephalomyelitis by driving Th1 differentiation via ZAP70/NF-κB signaling.
    Article Snippet: The membrane was blocked with 5% BSA in TBST for 1.5 h at 37°C, then incubated for 2 h at 37°C with primary antibodies diluted in TBST with 5% BSA, including: NF-kB p65 (Rabbit anti-NF-kB p65, 32536,Abcam; 1:1000), phospho-NF-kB p65 (Ser276/Ser536,76302, Abcam; 1:1000), phospho-IkBa (Ser176/180; AP0707,ABclonal; 1:1000), IkBa (Rabbit anti-IkBa, 4814S,Cell Signaling Technology; 1:1000), and housekeeping proteins b-actin (total protein control, Rabbit anti-b-actin, 700068-8F10,Zheng Neng; 1:5000) and Histone H3 (nuclear control, Mouse anti-H3, 250182, Zheng Neng; 1:1000). .. After washing, the membrane was incubated with a biotinylated goat anti-rabbit secondary antibody [Biotin-SP AffiniPure Goat AntiRabbit IgG (H + L), Jackson ImmunoResearch, USA; 1:200,000] for Frontiers in Immunology 05 1 h at 37°C, followed by 0.1 μg/mL peroxidase-conjugated streptavidin (Jackson ImmunoResearch, USA). .. Detection was performed using ECL chemiluminescence reagents (Amersham/GE Healthcare), and images were captured with a Vilber Lourmat TFX-35 WL transilluminator (France).

    Article Title: Early micro and nanoscopic responses of microglia to blood-brain barrier modulation by transcranial-focused ultrasound
    Article Snippet: Subsequently, the sections were incubated with a rabbit anti-Iba1 primary antibody (1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical, Virginia, USA) overnight at 4°C. .. The following day, after 3 consecutive TBS washes, the sections were incubated in a biotinylated goat anti-rabbit secondary antibody (1:300 in TBS, cat# 111-066-046, Jackson Immunoresearch, Philadelphia, USA) and subsequently in an avidin–biotin solution for 1 h. Staining was revealed with 0.05% 3-3′-diaminobenzidine (DAB, D5905-50TAB, Millipore Sigma, Massachusetts, USA) and 0.015% H 2 O 2 in 100 mM Tris–HCl. .. After mounting and drying, the sections were incubated in ultrapure H 2 O, increasing ethanol concentrations (50%, 70%, 80%, 90%, 100%) and xylene (cat# 534056, Millipore Sigma, Massachusetts, USA) for 5 min each at RT.

    Immunostaining:

    Article Title: Chronic electroconvulsive shock disrupts hippocampal physiology and impairs associative memory in mice
    Article Snippet: .. For free floating fluorescent immunostaining of c-fos, sections were incubated with 1% H 2 O 2 for 30min, rabbit anti-Fos antibody (1:1000; Synaptic Systems) for 3 days at 4°C, biotinylated goat anti-rabbit secondary antibody (Jackson Immunoresearch; 1:250) for 1 hour at room temperature, streptavidin-HRP for 1 hour and finally streptavidin-conjugated Alexa 647 (Thermofisher). ..

    Staining:

    Article Title: MCJ: A mitochondrial target for cardiac intervention in pulmonary hypertension
    Article Snippet: .. Human lung sections were stained with anti-MCJ antibody (EPR12823) (1:50; Abcam, no. ab179820) followed by a biotinylated goat anti-rabbit secondary antibody (1:500; Jackson ImmunoResearch Laboratories), streptavidin-conjugated avidin–biotin complex (ABC), and 3,3′-diaminobenzidene conjugated to HRP (Vector Laboratories, catalog no. PK-6100). .. Immunostained sections were briefly counterstained with Nuclear Fast Red hematoxylin (Sigma-Aldrich).

    Article Title: Early micro and nanoscopic responses of microglia to blood-brain barrier modulation by transcranial-focused ultrasound
    Article Snippet: Subsequently, the sections were incubated with a rabbit anti-Iba1 primary antibody (1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical, Virginia, USA) overnight at 4°C. .. The following day, after 3 consecutive TBS washes, the sections were incubated in a biotinylated goat anti-rabbit secondary antibody (1:300 in TBS, cat# 111-066-046, Jackson Immunoresearch, Philadelphia, USA) and subsequently in an avidin–biotin solution for 1 h. Staining was revealed with 0.05% 3-3′-diaminobenzidine (DAB, D5905-50TAB, Millipore Sigma, Massachusetts, USA) and 0.015% H 2 O 2 in 100 mM Tris–HCl. .. After mounting and drying, the sections were incubated in ultrapure H 2 O, increasing ethanol concentrations (50%, 70%, 80%, 90%, 100%) and xylene (cat# 534056, Millipore Sigma, Massachusetts, USA) for 5 min each at RT.

    Avidin-Biotin Assay:

    Article Title: MCJ: A mitochondrial target for cardiac intervention in pulmonary hypertension
    Article Snippet: .. Human lung sections were stained with anti-MCJ antibody (EPR12823) (1:50; Abcam, no. ab179820) followed by a biotinylated goat anti-rabbit secondary antibody (1:500; Jackson ImmunoResearch Laboratories), streptavidin-conjugated avidin–biotin complex (ABC), and 3,3′-diaminobenzidene conjugated to HRP (Vector Laboratories, catalog no. PK-6100). .. Immunostained sections were briefly counterstained with Nuclear Fast Red hematoxylin (Sigma-Aldrich).

    Article Title: Early micro and nanoscopic responses of microglia to blood-brain barrier modulation by transcranial-focused ultrasound
    Article Snippet: Subsequently, the sections were incubated with a rabbit anti-Iba1 primary antibody (1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical, Virginia, USA) overnight at 4°C. .. The following day, after 3 consecutive TBS washes, the sections were incubated in a biotinylated goat anti-rabbit secondary antibody (1:300 in TBS, cat# 111-066-046, Jackson Immunoresearch, Philadelphia, USA) and subsequently in an avidin–biotin solution for 1 h. Staining was revealed with 0.05% 3-3′-diaminobenzidine (DAB, D5905-50TAB, Millipore Sigma, Massachusetts, USA) and 0.015% H 2 O 2 in 100 mM Tris–HCl. .. After mounting and drying, the sections were incubated in ultrapure H 2 O, increasing ethanol concentrations (50%, 70%, 80%, 90%, 100%) and xylene (cat# 534056, Millipore Sigma, Massachusetts, USA) for 5 min each at RT.



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